Haworth R A, Hunter D R, Berkoff H A
Arch Biochem Biophys. 1984 Aug 15;233(1):106-14. doi: 10.1016/0003-9861(84)90606-4.
3-O-Methylglucose uptake by Ca2+-resistant adult rat heart cells in suspension was measured, free of artifactual inhibitor-insensitive uptake, and with an accuracy of +/- 1.9% pellet water. (Ca2+-resistant cells are cells which retain their original rod-shaped morphology in the presence of physiological levels of Ca2+.) High levels of insulin (10(-6) M) stimulated the rate of 3-O-methylglucose uptake approximately 10-fold. In the presence of low levels of insulin (3 X 10(-11) M, 10(-10) M) uptake was biphasic; it could not be described by a single exponential function within experimental error, but required the sum of two exponentials. Deviation from a single exponential function was not so great with high levels of insulin (10(-6) M) or no insulin. Cell sugar uptake was also investigated using autoradiography of cells which had accumulated [2-14C]deoxyglucose under similar conditions. This showed considerable heterogeneity of 2-deoxyglucose uptake by cells treated with low levels of insulin, but significantly less heterogeneity of 2-deoxyglucose uptake by cells treated with high levels of insulin. It is concluded that the deviation of 3-O-methylglucose uptake from a single exponential observed at low insulin levels can be accounted for in terms of a heterogeneous response of cells to insulin.
对悬浮状态下成年大鼠抗钙心肌细胞摄取3 - O - 甲基葡萄糖的情况进行了测量，测量过程无假象的非抑制剂敏感性摄取，测量精度为±1.9%的沉淀水。（抗钙细胞是指在生理水平的钙离子存在下仍保持其原始杆状形态的细胞。）高浓度胰岛素（10⁻⁶ M）使3 - O - 甲基葡萄糖摄取速率增加约10倍。在低浓度胰岛素（3×10⁻¹¹ M、10⁻¹⁰ M）存在时，摄取呈双相性；在实验误差范围内，无法用单一指数函数描述，而是需要两个指数函数之和。高浓度胰岛素（10⁻⁶ M）或无胰岛素时，与单一指数函数的偏差没那么大。还使用了在类似条件下积累了[2 - ¹⁴C]脱氧葡萄糖的细胞的放射自显影技术研究细胞对糖的摄取。这表明，低浓度胰岛素处理的细胞对2 - 脱氧葡萄糖的摄取存在相当大的异质性，但高浓度胰岛素处理的细胞对2 - 脱氧葡萄糖的摄取异质性明显较小。得出的结论是，低胰岛素水平下观察到的3 - O - 甲基葡萄糖摄取偏离单一指数函数的情况，可以用细胞对胰岛素的异质性反应来解释。
