Holub B J
Can J Biochem Cell Biol. 1984 Jun;62(6):341-51. doi: 10.1139/o84-048.
Extensive research during the past few years has indicated that dramatic alterations in phospholipid metabolism and composition represent early and important biochemical events in the response of human platelets to thrombin stimulation. The individual enzyme-catalyzed steps which provide for the release of free arachidonic acid for thromboxane A2 formation via the initial degradation of phosphatidylcholine and phosphatidylinositol have been studied. Their importance in this regard is influenced by the molecular species composition of the corresponding phospholipid precursors. A role for stimulated phosphatidylinositol 4,5-bisphosphate degradation in the phosphatidylinositol response and inositol triphosphate release associated with calcium mobilization has also been proposed. The 1,2-diacylglycerol released by the action of phospholipase C on phosphatidylinositol and its 4,5-bisphosphate derivative has been implicated as an activator of protein phosphorylation; the derived phosphatidic acid has been proposed as a mediator for promoting an intracellular flux of calcium associated with platelet responses.
过去几年的大量研究表明，磷脂代谢和组成的显著变化是人类血小板对凝血酶刺激反应中早期且重要的生化事件。已经研究了通过磷脂酰胆碱和磷脂酰肌醇的初始降解为血栓素A2形成提供游离花生四烯酸释放的各个酶催化步骤。它们在这方面的重要性受到相应磷脂前体分子种类组成的影响。还提出了刺激的磷脂酰肌醇4,5 - 二磷酸降解在磷脂酰肌醇反应以及与钙动员相关的肌醇三磷酸释放中的作用。磷脂酶C作用于磷脂酰肌醇及其4,5 - 二磷酸衍生物释放的1,2 - 二酰基甘油被认为是蛋白质磷酸化的激活剂；衍生的磷脂酸被认为是促进与血小板反应相关的细胞内钙通量的介质。
