Agwu D E, Johnstone I, Crane S, Holub B J
Lipids. 1983 Dec;18(12):859-62. doi: 10.1007/BF02534562.
The degradation of platelet phospholipids via phospholipase activity is known to occur during thrombin-induced platelet aggregation. Both phosphatidylinositol and phosphatidylcholine are considered to be sources of the released arachidonic acid which becomes a substrate for prostaglandin and thromboxane A2 formation. In this work, the effect of thrombin on the incorporation of exogenous arachidonic acid into platelet membrane phospholipids was studied. Suspensions of gerbil platelets were incubated in aggregometer cuvettes with [14C] arachidonic acid in the absence or presence of thrombin, and product formation was monitored by thin layer chromatography and scintillation counting. Within 30 sec, the entry of arachidonic acid into phosphatidylinositol was increased by 165% in thrombin-stimulated platelets over controls. Under identical conditions, the incorporation into phosphatidylcholine was increased by only 57%. These results suggest that the incorporation of exogenous arachidonic acid via lysophosphatidylinositol and lysophosphatidylcholine acyltransferase activities may be intimately associated with thrombin-induced platelet aggregation in the gerbil.
已知在凝血酶诱导的血小板聚集过程中，会通过磷脂酶活性发生血小板磷脂的降解。磷脂酰肌醇和磷脂酰胆碱都被认为是释放的花生四烯酸的来源，花生四烯酸会成为前列腺素和血栓素A2形成的底物。在这项研究中，研究了凝血酶对外源性花生四烯酸掺入血小板膜磷脂的影响。将沙鼠血小板悬浮液在聚集仪比色杯中与[14C]花生四烯酸一起孵育，有无凝血酶存在，通过薄层色谱法和闪烁计数监测产物形成。在30秒内，与对照相比，凝血酶刺激的血小板中花生四烯酸进入磷脂酰肌醇的量增加了165%。在相同条件下，掺入磷脂酰胆碱的量仅增加了57%。这些结果表明，通过溶血磷脂酰肌醇和溶血磷脂酰胆碱酰基转移酶活性掺入外源性花生四烯酸可能与沙鼠中凝血酶诱导的血小板聚集密切相关。
