Miller S P, Marinkovich V A, Riege D H, Sell W J, Baker D L, Eldredge N T, Dyminski J W, Hale R G, Peisach J M, Burd J F
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The MAST Immunodiagnostic Test System was developed to provide a comprehensive, simple means for the in vitro measurement of multiple antigens or antibodies. The first commercial application of the MAST system incorporates several novel features for cost-effective diagnosis of IgE-mediated allergy in a clinical laboratory or a physician's office. The basis of the MAST system is a unique analytical test chamber, which contains cellulose thread as the solid-phase matrix and allows multiple test results from a single assay. This test chamber incorporates both positive and negative controls and requires no volume-dependent pipetting steps. Immunographic exposure onto high-speed Polaroid instant film allows for quantifying results with an automatic recording infrared-transmittance densitometer. Test results are easily interpreted by using a patient test record provided with the system. The MAST system greatly simplifies testing for allergen-specific IgE, while retaining specificity and sensitivity. Currently, with the MAST system one can simultaneously measure picomoles of allergen-specific IgE in up to 35 different allergen classes. In addition to allergy testing, the MAST technology is applicable to other immunodiagnostic profiles.
MAST免疫诊断测试系统的开发目的是提供一种全面、简单的体外检测多种抗原或抗体的方法。MAST系统的首次商业应用包含了几个新颖的特性，可在临床实验室或医生办公室对IgE介导的过敏进行经济高效的诊断。MAST系统的基础是一个独特的分析测试腔，它以纤维素线作为固相基质，一次检测就能得出多个测试结果。该测试腔包含阳性和阴性对照，无需依赖体积的移液步骤。在高速宝丽来即时胶片上进行免疫显影，可通过自动记录红外透射密度计对结果进行定量。使用该系统提供的患者测试记录，测试结果很容易解读。MAST系统极大地简化了过敏原特异性IgE的检测，同时保持了特异性和灵敏度。目前，使用MAST系统可以同时检测多达35种不同过敏原类别的皮摩尔级过敏原特异性IgE。除了过敏检测外，MAST技术还适用于其他免疫诊断分析。
