Glauert H P, Reddy J K, Kennan W S, Sattler G L, Rao V S, Pitot H C
Cancer Lett. 1984 Sep;24(2):147-56. doi: 10.1016/0304-3835(84)90130-7.
The peroxisome proliferators Wy-14,643, BR-931, nafenopin and ciprofibrate were tested in the primary hepatocyte culture-unscheduled DNA synthesis assay and in the Ames Salmonella microsome mutagenicity assay. The amount of unscheduled DNA synthesis (UDS) in hepatocytes was determined by quantifying the amount of [3H]thymidine incorporated into DNA in the presence of hydroxyurea after isolation of nuclei from hepatocytes treated with the test agent. Wy-14,643 and BR-931 induced unscheduled DNA synthesis in rat hepatocytes, whereas nafenopin and ciprofibrate had no effect. All of the peroxisome proliferators were negative in the Ames Salmonella assay.
在原代肝细胞培养非程序性DNA合成试验和艾姆斯沙门氏菌微粒体诱变性试验中，对过氧化物酶体增殖剂Wy-14,643、BR-931、萘酚平及环丙贝特进行了测试。在用受试药物处理肝细胞后，分离细胞核，通过定量在羟基脲存在的情况下掺入DNA中的[3H]胸腺嘧啶核苷的量，来测定肝细胞中非程序性DNA合成（UDS）的量。Wy-14,643和BR-931可诱导大鼠肝细胞发生非程序性DNA合成，而萘酚平和环丙贝特则无此作用。在艾姆斯沙门氏菌试验中，所有过氧化物酶体增殖剂均呈阴性。
