Kwan H S, Barrett E L
J Bacteriol. 1984 Sep;159(3):1090-2. doi: 10.1128/jb.159.3.1090-1092.1984.
Menaquinone (men) mutants of Salmonella typhimurium isolated on the basis of their inability to produce trimethylamine were characterized with respect to mutation site, the ability to cross-feed each other and be cross-fed by known Escherichia coli men mutants, and response to intermediates of the menaquinone biosynthetic pathway. Cross-feeding tests were based on the requirement of menaquinone for hydrogen sulfide production. Genotypes corresponding to the menA, B, C, D, and possibly E genes described in E. coli were all identified. Additional studies of deletions in the menBCD area revealed that this cluster lies between ack/pta and glpT, as in E. coli. The ack and pta mutants were also defective in the production of trimethylamine and failed to produce gas in the absence of added formate.
基于无法产生三甲胺而分离出的鼠伤寒沙门氏菌甲萘醌（men）突变体，在突变位点、相互交叉喂养以及被已知大肠杆菌men突变体交叉喂养的能力，以及对甲萘醌生物合成途径中间体的反应方面进行了表征。交叉喂养试验基于甲萘醌对硫化氢产生的需求。鉴定出了与大肠杆菌中描述的menA、B、C、D以及可能的E基因相对应的基因型。对menBCD区域缺失的进一步研究表明，该基因簇位于ack/pta和glpT之间，与大肠杆菌中的情况相同。ack和pta突变体在三甲胺产生方面也存在缺陷，并且在不添加甲酸盐的情况下无法产生气体。
