大肠杆菌和鼠伤寒沙门氏菌乙酸激酶及磷酸转乙酰酶突变体的分离与鉴定
Isolation and Characterization of acetate kinase and phosphotransacetylase mutants of Escherichia coli and Salmonella typhimurium.
作者信息
LeVine S M, Ardeshir F, Ames G F
出版信息
J Bacteriol. 1980 Aug;143(2):1081-5. doi: 10.1128/jb.143.2.1081-1085.1980.
Abstract
Mutations in the ack (acetate kinase) and pta (phosphotransacetylase) genes in Salmonella typhimurium were characterized and determined to be analogous to those of previously described Escherichia coli mutants. We established that in both bacterial species these genes were cotransducible with the neighboring histidine transport operon and were distally located relative to purF. pta mutants were sensitive to the dye alizarin yellow and were unable to grow on medium containing inositol as a carbon source. We selected mutants of both species with deletions covering both the ack and the pta genes; some deletions extended into the histidine transport operon.
摘要
对鼠伤寒沙门氏菌中ack（乙酸激酶）和pta（磷酸转乙酰酶）基因的突变进行了表征，并确定它们与先前描述的大肠杆菌突变体类似。我们确定，在这两种细菌中，这些基因与相邻的组氨酸转运操纵子共转导，并且相对于purF位于远端。pta突变体对染料茜素黄敏感，并且无法在含有肌醇作为碳源的培养基上生长。我们筛选了这两种细菌的突变体，其缺失覆盖了ack和pta基因；一些缺失延伸到组氨酸转运操纵子中。
相似文献
1
Isolation and Characterization of acetate kinase and phosphotransacetylase mutants of Escherichia coli and Salmonella typhimurium.大肠杆菌和鼠伤寒沙门氏菌乙酸激酶及磷酸转乙酰酶突变体的分离与鉴定
J Bacteriol. 1980 Aug;143(2):1081-5. doi: 10.1128/jb.143.2.1081-1085.1980.
2
Involvement of ack-pta operon products in alpha-ketobutyrate metabolism by Salmonella typhimurium.鼠伤寒沙门氏菌中ack-pta操纵子产物参与α-酮丁酸代谢
Mol Gen Genet. 1987 May;207(2-3):435-40. doi: 10.1007/BF00331612.
3
Identification and characterization of the ackA (acetate kinase A)-pta (phosphotransacetylase) operon and complementation analysis of acetate utilization by an ackA-pta deletion mutant of Escherichia coli.大肠杆菌ackA（乙酸激酶A）-pta（磷酸转乙酰酶）操纵子的鉴定与表征以及ackA-pta缺失突变体对乙酸利用的互补分析
J Biochem. 1994 Oct;116(4):916-22. doi: 10.1093/oxfordjournals.jbchem.a124616.
4
Cloning, sequence analysis, expression and inactivation of the Corynebacterium glutamicum pta-ack operon encoding phosphotransacetylase and acetate kinase.谷氨酸棒杆菌编码磷酸转乙酰酶和乙酸激酶的pta-ack操纵子的克隆、序列分析、表达及失活
Microbiology (Reading). 1999 Feb;145 ( Pt 2):503-513. doi: 10.1099/13500872-145-2-503.
5
Cloning of a gene coding for phosphotransacetylase from Escherichia coli.来自大肠杆菌的磷酸转乙酰酶编码基因的克隆。
Appl Microbiol Biotechnol. 1990 Sep;33(6):680-2. doi: 10.1007/BF00604937.
6
ack::Mu d1-8 (Apr lac) operon fusions of Salmonella typhimurium LT2.鼠伤寒沙门氏菌LT2的ack::Mu d1-8（Apr lac）操纵子融合体
Mol Gen Genet. 1988 Jan;211(1):183-5. doi: 10.1007/BF00338411.
7
Construction of Pta-Ack pathway deletion mutants of Escherichia coli and characteristic growth profiles of the mutants in a rich medium.大肠杆菌Pta-Ack途径缺失突变体的构建及其在丰富培养基中的特征生长曲线
Biosci Biotechnol Biochem. 1994 Dec;58(12):2232-5. doi: 10.1271/bbb.58.2232.
8
Cloning, sequence analysis, and hyperexpression of the genes encoding phosphotransacetylase and acetate kinase from Methanosarcina thermophila.嗜热甲烷八叠球菌磷酸转乙酰酶和乙酸激酶编码基因的克隆、序列分析及高效表达
J Bacteriol. 1993 Nov;175(21):6822-9. doi: 10.1128/jb.175.21.6822-6829.1993.
9
Cloning of the histidine transport genes from Salmonella typhimurium and characterization of an analogous transport system in Escherichia coli.鼠伤寒沙门氏菌组氨酸转运基因的克隆及大肠杆菌中类似转运系统的特性研究。
J Supramol Struct. 1980;13(1):117-30. doi: 10.1002/jss.400130111.
10
The enzymic interconversion of acetate and acetyl-coenzyme A in Escherichia coli.大肠杆菌中乙酸盐与乙酰辅酶A的酶促相互转化
J Gen Microbiol. 1977 Oct;102(2):327-36. doi: 10.1099/00221287-102-2-327.
引用本文的文献
1
Evidence that a metabolic microcompartment contains and recycles private cofactor pools.证据表明，代谢微区室包含并循环使用私有辅因子池。
J Bacteriol. 2013 Jun;195(12):2864-79. doi: 10.1128/JB.02179-12. Epub 2013 Apr 12.
2
Elimination of D-lactate synthesis increases poly(3-hydroxybutyrate) and ethanol synthesis from glycerol and affects cofactor distribution in recombinant Escherichia coli.消除 D-乳酸合成可增加甘油合成聚（3-羟基丁酸酯）和乙醇，并影响重组大肠杆菌中辅酶的分布。
Appl Environ Microbiol. 2010 Nov;76(22):7400-6. doi: 10.1128/AEM.02067-10. Epub 2010 Sep 24.
3
PduL is an evolutionarily distinct phosphotransacylase involved in B12-dependent 1,2-propanediol degradation by Salmonella enterica serovar typhimurium LT2.PduL是一种在进化上独特的磷酸转酰酶，参与鼠伤寒沙门氏菌LT2依赖维生素B12的1,2-丙二醇降解过程。
J Bacteriol. 2007 Mar;189(5):1589-96. doi: 10.1128/JB.01151-06. Epub 2006 Dec 8.
4
Paired termini stabilize antisense RNAs and enhance conditional gene silencing in Escherichia coli.配对末端可稳定反义RNA并增强大肠杆菌中的条件性基因沉默。
Nucleic Acids Res. 2006;34(20):e138. doi: 10.1093/nar/gkl697. Epub 2006 Oct 24.
5
Cellulase, clostridia, and ethanol.纤维素酶、梭菌与乙醇。
Microbiol Mol Biol Rev. 2005 Mar;69(1):124-54. doi: 10.1128/MMBR.69.1.124-154.2005.
6
Expression of an anaplerotic enzyme, pyruvate carboxylase, improves recombinant protein production in Escherichia coli.一种回补酶——丙酮酸羧化酶的表达可提高大肠杆菌中重组蛋白的产量。
Appl Environ Microbiol. 2002 Nov;68(11):5620-4. doi: 10.1128/AEM.68.11.5620-5624.2002.
7
Acetate and formate stress: opposite responses in the proteome of Escherichia coli.乙酸盐和甲酸盐胁迫：大肠杆菌蛋白质组中的相反反应。
J Bacteriol. 2001 Nov;183(21):6466-77. doi: 10.1128/JB.183.21.6466-6477.2001.
8
Requirement for the enzymes acetoacetyl coenzyme A synthetase and poly-3-hydroxybutyrate (PHB) synthase for growth of Sinorhizobium meliloti on PHB cycle intermediates.苜蓿中华根瘤菌利用聚-3-羟基丁酸（PHB）循环中间体生长对乙酰乙酰辅酶A合成酶和聚-3-羟基丁酸（PHB）合酶的需求。
J Bacteriol. 2000 Apr;182(8):2113-8. doi: 10.1128/JB.182.8.2113-2118.2000.
9
RpoS synthesis is growth rate regulated in Salmonella typhimurium, but its turnover is not dependent on acetyl phosphate synthesis or PTS function.鼠伤寒沙门氏菌中RpoS的合成受生长速率调控，但其周转并不依赖于乙酰磷酸的合成或磷酸转移酶系统（PTS）功能。
J Bacteriol. 1999 Aug;181(16):4853-62. doi: 10.1128/JB.181.16.4853-4862.1999.
10
Linkage map of Escherichia coli K-12, edition 10: the traditional map.大肠杆菌K-12连锁图谱，第10版：传统图谱。
Microbiol Mol Biol Rev. 1998 Sep;62(3):814-984. doi: 10.1128/MMBR.62.3.814-984.1998.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Enzymatic phosphorylation of acetate.乙酸的酶促磷酸化作用。
J Biol Chem. 1954 Dec;211(2):737-56.
3
Effect of the alpha-hydrazino analogue of histidine on histidine transport and arginine biosynthesis.组氨酸的α-肼基类似物对组氨酸转运及精氨酸生物合成的影响。
J Biol Chem. 1966 Jul 25;241(14):3424-9.
4
Compounds which serve as the sole source of carbon or nitrogen for Salmonella typhimurium LT-2.作为鼠伤寒沙门氏菌LT-2唯一碳源或氮源的化合物。
J Bacteriol. 1969 Oct;100(1):215-9. doi: 10.1128/jb.100.1.215-219.1969.
5
The inhibition of acetate, pyruvate, and 3-phosphogylcerate kinases by chromium adenosine triphosphate.三磷酸腺苷铬对乙酸激酶、丙酮酸激酶和3-磷酸甘油酸激酶的抑制作用。
J Biol Chem. 1974 Apr 25;249(8):2567-71.
6
7
Recalibrated linkage map of Escherichia coli K-12.大肠杆菌K-12的重新校准连锁图谱。
Bacteriol Rev. 1976 Mar;40(1):116-67. doi: 10.1128/br.40.1.116-167.1976.
8
Anaerobic growth of Escherichia coli K12 with fumarate as terminal electron acceptor. Genetic studies with menaquinone and fluoroacetate-resistant mutants.以富马酸为末端电子受体的大肠杆菌K12厌氧生长。对甲基萘醌和氟乙酸抗性突变体的遗传学研究。
J Gen Microbiol. 1979 Dec;115(2):259-71. doi: 10.1099/00221287-115-2-259.
9
Requirements of acetyl phosphate for the binding protein-dependent transport systems in Escherichia coli.乙酰磷酸对大肠杆菌中依赖结合蛋白的转运系统的需求
Proc Natl Acad Sci U S A. 1979 Mar;76(3):1213-7. doi: 10.1073/pnas.76.3.1213.
10
Evidence for a common mechanism for the insertion of the Tn10 transposon and for the generation of Tn10-stimulated deletions.
Mol Gen Genet. 1978 Oct 30;166(2):217-23. doi: 10.1007/BF00285924.
Zotero 插件