Morrison N A, Cen Y H, Chen H C, Plazinski J, Ridge R, Rolfe B G
J Bacteriol. 1984 Oct;160(1):483-7. doi: 10.1128/jb.160.1.483-487.1984.
A large Sym plasmid from a fast-growing cowpea Rhizobium species was made mobilizable by cointegration with plasmid pSUP1011, which carries the oriT region of RP4. This mobilizable Sym plasmid was transferred to a number of Rhizobium strains, in which nodulation and nitrogen fixation functions for symbiosis with plants of the cowpea group were expressed.
通过与携带RP4的oriT区域的质粒pSUP1011共整合,使来自快速生长的豇豆根瘤菌物种的一个大型共生质粒变得可转移。这个可转移的共生质粒被转移到多个根瘤菌菌株中,在这些菌株中表达了与豇豆组植物共生的结瘤和固氮功能。