Cogen R B, Al-Joburi W, Gantt D G, Denys F R
J Clin Periodontol. 1984 Sep;11(8):531-9. doi: 10.1111/j.1600-051x.1984.tb00905.x.
Human teeth extracted because of advanced periodontal disease were obtained. The portions of the roots which had been exposed in periodontal pockets were either untreated or were treated with root planing or citric acid, or root planing followed by citric acid. Human gingival fibroblasts were then added to the roots so treated and were allowed to incubate for 72 h. The ability of cells to attach to and grow onto these roots was assessed by means of gross evaluation of staining intensity and by histologic and scanning electron microscopic observation. The results of multiple experiments in each root-treatment category indicated that only roots which had been planed, whether or not citric acid demineralization was used, promoted cell attachment and growth. In addition, there were no discernible morphologic differences in the cells which were plated onto roots which were root planed only, compared to those which were root planed and citric-acid treated. In both situations too, the cells displayed morphology typical of human gingival fibroblasts in culture.
获取因晚期牙周病而拔除的人类牙齿。将暴露于牙周袋中的牙根部分要么不进行处理，要么进行根面平整或用柠檬酸处理，或者先进行根面平整再用柠檬酸处理。然后将人类牙龈成纤维细胞添加到经过上述处理的牙根上，并使其孵育72小时。通过对染色强度的总体评估以及组织学和扫描电子显微镜观察来评估细胞附着于这些牙根并在其上生长的能力。每个牙根处理类别的多次实验结果表明，只有经过根面平整的牙根，无论是否使用柠檬酸脱矿处理，都能促进细胞附着和生长。此外，接种到仅经过根面平整的牙根上的细胞与经过根面平整和柠檬酸处理的牙根上的细胞相比，在形态上没有明显差异。在这两种情况下，细胞均呈现出培养中人类牙龈成纤维细胞的典型形态。
