Shenep J L, Mogan K A
J Infect Dis. 1984 Sep;150(3):380-8. doi: 10.1093/infdis/150.3.380.
For study of the kinetics of endotoxin release from bacterial cells during therapy for gram-negative bacterial sepsis, serial blood samples were obtained from rabbits with Escherichia coli sepsis that were treated with either antibiotic or placebo. The concentrations of viable bacteria, free endotoxin, and total endotoxin in each blood sample were quantitated. In animals treated with placebo the concentration of free endotoxin was proportional to the level of bacteremia. In contrast, in animals treated with antibiotic the plasma levels of free endotoxin increased 10- to 2,000-fold, in spite of decreasing levels of bacteremia. Free endotoxin that was present in the plasma following antibiotic treatment appeared to be derived in part from the breakdown of circulating bacteria and in part from the disintegration of bacteria in tissues other than the blood. The results of this study demonstrate that significant amounts of endotoxin are released from bacterial cells following administration of antibiotics in vivo.
为研究革兰氏阴性菌败血症治疗期间细菌细胞内毒素释放的动力学，从患有大肠杆菌败血症且接受抗生素或安慰剂治疗的兔子身上采集系列血样。对每份血样中的活菌、游离内毒素和总内毒素浓度进行定量。接受安慰剂治疗的动物中，游离内毒素浓度与菌血症水平成正比。相比之下，接受抗生素治疗的动物，尽管菌血症水平下降，但血浆中游离内毒素水平却升高了10至2000倍。抗生素治疗后血浆中存在的游离内毒素似乎部分源自循环细菌的分解，部分源自血液以外组织中细菌的解体。本研究结果表明，体内给予抗生素后，细菌细胞会释放大量内毒素。
