Collins F M, Auclair L K
J Leukoc Biol. 1984 Sep;36(3):321-32. doi: 10.1002/jlb.36.3.321.
Specific pathogen-free LBN rats were parabiotically linked and the monocyte donor animal was labeled with multiple pulses of tritiated thymidine (1 microCi/g body weight). The right-hand (recipient) rat lungs were infected with 10(5) viable Mycobacterium bovis (BCG) Pasteur by the intravenous, aerogenic, or intratracheal routes. Control animals received heat-killed BCG or saline only, given intratracheally. The BCG infection resulted in a ten-fold increase in the number of heavily labeled, blood-derived monocytes recovered 24 hr later in the lung lavage fluid. The percentage of labeled cells peaked on day 3 and then declined slowly. Introduction of heat-killed BCG into the lung produced a smaller mononuclear cell influx but a marked polymorphonuclear phagocyte response that persisted for several days. The labeled monocyte counts for the infected recipient rat lung washouts were five to ten times those for the uninfected donor parabiont, except when the aerogenic infection route was used, when both donor and recipient rats were equally infected and both showed substantial increases in labeled monocytes in the lung washouts.
将无特定病原体的LBN大鼠进行联体手术，单核细胞供体动物用多次氚化胸腺嘧啶脉冲（1微居里/克体重）标记。右手侧（受体）大鼠的肺部通过静脉内、气溶胶或气管内途径感染10⁵ 活的牛分枝杆菌（卡介苗）巴斯德株。对照动物仅经气管内给予热灭活卡介苗或生理盐水。卡介苗感染导致24小时后肺灌洗液中回收的高度标记的、源自血液的单核细胞数量增加了10倍。标记细胞的百分比在第3天达到峰值，然后缓慢下降。将热灭活卡介苗引入肺部产生的单核细胞流入较少，但多形核吞噬细胞反应明显，持续数天。感染受体大鼠肺冲洗液中的标记单核细胞计数是未感染供体联体动物的五到十倍，除非采用气溶胶感染途径，此时供体和受体大鼠均受到同等感染，且两者肺冲洗液中的标记单核细胞均显著增加。
