Johnston G I, Heptinstall S, Robins R A, Price M R
Biochem Biophys Res Commun. 1984 Sep 28;123(3):1091-8. doi: 10.1016/s0006-291x(84)80245-4.
Glycoproteins present on the surface of blood platelets are fundamental to normal blood platelet behaviour. We have used monoclonal antibodies and flow cytofluorimetry to study the expression of glycoproteins on single platelets from normal subjects, and from patients with Glanzmann's thrombasthenia and the Bernard-Soulier syndrome. We show that normal platelets are heterogeneous in that individual cells display markedly different numbers of glycoprotein IIb/IIIa complex and glycoprotein Ib molecules. We also show that the two congenital bleeding disorders are associated with markedly reduced numbers of glycoprotein IIb/IIIa complex or glycoprotein Ib molecules on all the platelets rather than the difference residing in a sub-population.
血小板表面的糖蛋白对正常血小板行为至关重要。我们使用单克隆抗体和流式细胞荧光测定法研究了正常受试者、血小板无力症患者和Bernard-Soulier综合征患者单个血小板上糖蛋白的表达。我们发现正常血小板具有异质性，因为单个细胞上糖蛋白IIb/IIIa复合物和糖蛋白Ib分子的数量明显不同。我们还表明，这两种先天性出血性疾病与所有血小板上糖蛋白IIb/IIIa复合物或糖蛋白Ib分子数量的显著减少有关，而不是存在于亚群中的差异。
