Ronner J A, Lensen H G, Olthuis F M, Smolders C A, Feijen J
Biomaterials. 1984 Jul;5(4):241-3. doi: 10.1016/0142-9612(84)90023-1.
The adsorption behaviour of two commercial preparations of human IgG onto a polystyrene latex surface was studied. The adsorption isotherms obtained differed markedly; one preparation showed a plateau value of 0.4 microgram cm-2 which was reached at 0.1 g l-1, whereas the other preparation showed no plateau value within the concentration range studied (0.1-7.0 g l-1). Characterization by means of iso-electric focusing and HPLC also showed differences between the two preparations. No differences were observed when immuno-electrophoresis was carried out. These results stress the necessity for proper characterization of proteins used in adsorption studies.
研究了两种市售人免疫球蛋白（IgG）制剂在聚苯乙烯胶乳表面的吸附行为。得到的吸附等温线有显著差异；一种制剂在0.1 g l-1时达到0.4微克 cm-2的平稳值，而另一种制剂在所研究的浓度范围（0.1 - 7.0 g l-1）内未显示平稳值。通过等电聚焦和高效液相色谱法进行的表征也显示两种制剂之间存在差异。进行免疫电泳时未观察到差异。这些结果强调了在吸附研究中对所用蛋白质进行适当表征的必要性。
