用于检测肺炎链球菌抗原的酶联免疫吸附测定
Enzyme-linked immunosorbent assay for detection of Streptococcus pneumoniae antigen.
作者信息
Harding S A, Scheld W M, McGowan M D, Sande M A
出版信息
J Clin Microbiol. 1979 Sep;10(3):339-42. doi: 10.1128/jcm.10.3.339-342.1979.
Abstract
An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of Streptococcus pneumoniae polysaccharide antigen in cerebrospinal fluid and serum. Sensitivity and specificity were determined for purified antigen preparations. Specificity was also evaluated in the rabbit meningitis model, and the sensitivity was compared to counterimmunoelectrophoresis, using the infected rabbits' cerebrospinal fluid and serum. The ELISA was a specific technique for detecting S. pneumoniae antigen. ELISA was 25 times more sensitive than counterimmunoelectrophoresis for purified antigen and resulted in an increased positivity of the cerebrospinal fluid and serum from infected rabbits. ELISA should prove very useful in the diagnosis of pneumococcal infections.
摘要
开发了一种酶联免疫吸附测定法（ELISA），用于检测脑脊液和血清中的肺炎链球菌多糖抗原。测定了纯化抗原制剂的敏感性和特异性。还在兔脑膜炎模型中评估了特异性，并使用感染兔的脑脊液和血清，将敏感性与对流免疫电泳进行了比较。ELISA是检测肺炎链球菌抗原的一种特异性技术。对于纯化抗原，ELISA的敏感性比对流免疫电泳高25倍，并且使感染兔的脑脊液和血清的阳性率增加。ELISA在肺炎球菌感染的诊断中应会证明非常有用。
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