用于检测脑脊液中细菌抗原的Phadebact协同凝集试验与对流免疫电泳的比较。
Comparison of Phadebact coagglutination tests with counterimmunoelectrophoresis for the detection of bacterial antigens in cerebrospinal fluid.
作者信息
Tompkins D S
出版信息
J Clin Pathol. 1983 Jul;36(7):819-22. doi: 10.1136/jcp.36.7.819.
Abstract
One hundred and seventeen specimens of cerebrospinal fluid from 94 patients were examined for the presence of pneumococcal and Haemophilus influenzae type b antigens using counterimmunoelectrophoresis and coagglutination tests. The coagglutination method using Phadebact reagents was as sensitive as counterimmunoelectrophoresis, but culture was a more sensitive diagnostic procedure than either test. A meningococcus coagglutination reagent, included in a prototype meningitis diagnostic kit, was also found to be as sensitive as counterimmunoelectrophoresis when tested on culture-positive cerebrospinal fluid specimens. Coagglutination tests for the detection of bacterial antigen are useful supportive tests when used in conjunction with direct microscopy and culture for bacterial pathogens.
摘要
使用对流免疫电泳和协同凝集试验，对94例患者的117份脑脊液标本进行了肺炎球菌和b型流感嗜血杆菌抗原检测。使用法德巴克试剂的协同凝集法与对流免疫电泳一样灵敏，但培养是比这两种检测更灵敏的诊断方法。在一份脑膜炎诊断试剂盒原型中包含的一种脑膜炎球菌协同凝集试剂，在对培养阳性的脑脊液标本进行检测时，也被发现与对流免疫电泳一样灵敏。用于检测细菌抗原的协同凝集试验，与细菌病原体的直接显微镜检查和培养联合使用时，是有用的辅助检测方法。
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