Giraud A, Jönsson A C, Dockray G J
Peptides. 1984 Jul-Aug;5(4):757-63. doi: 10.1016/0196-9781(84)90018-4.
Antisera to the enkephalin variants Met-enk Arg6Phe7 and Met-enk Arg6Gly7Leu8 have been used in immunohistochemistry and radioimmunoassay studies of hog stomach. In the mucosa of the antrum, but not fundus, there was identified a population of immunoreactive endocrine-like cells. When extracts of antral mucosa were fractionated by gel filtration on Sephadex G50 the predominant immunoreactive forms of Met-enk Arg6Phe7 and Met-enk Arg6Gly7Leu8 were found to elute before the standards, and were compatible with N-terminally extended variants. In the muscle layers of both antrum and fundus, immunoreactive nerve fibers were found, these were especially numerous in the myenteric plexus. In extracts of the antral muscle, 50-60% of both Met-enk Arg6Phe7 and Met-enk Arg6Gly7Leu8 immunoreactivity eluted in the position of the standards and the remainder had the properties of N-terminally extended variants. In the fundus muscle the variants accounted for 70-80% of total activity. The results indicate that the proenkephalin gene is expressed in neurones and endocrine cells of the hog stomach. The different patterns of molecular forms found in different regions of the stomach suggest that the precursor is processed by different pathways in different populations of endocrine cells and neurones.
针对脑啡肽变体甲硫氨酸脑啡肽-精氨酸6-苯丙氨酸7（Met-enk Arg6Phe7）和甲硫氨酸脑啡肽-精氨酸6-甘氨酸7-亮氨酸8（Met-enk Arg6Gly7Leu8）的抗血清已用于猪胃的免疫组织化学和放射免疫分析研究。在胃窦而非胃底的黏膜中，鉴定出一群免疫反应性内分泌样细胞。当胃窦黏膜提取物在葡聚糖凝胶G50上进行凝胶过滤分级分离时，发现甲硫氨酸脑啡肽-精氨酸6-苯丙氨酸7和甲硫氨酸脑啡肽-精氨酸6-甘氨酸7-亮氨酸8的主要免疫反应形式在标准品之前洗脱，并且与N端延伸变体相符。在胃窦和胃底的肌层中，发现了免疫反应性神经纤维，这些在肌间神经丛中尤其丰富。在胃窦肌层提取物中，甲硫氨酸脑啡肽-精氨酸6-苯丙氨酸7和甲硫氨酸脑啡肽-精氨酸6-甘氨酸7-亮氨酸8的免疫反应性有50%-60%在标准品位置洗脱，其余部分具有N端延伸变体的特性。在胃底肌层中，这些变体占总活性的70%-80%。结果表明，脑啡肽原基因在猪胃的神经元和内分泌细胞中表达。在胃的不同区域发现的不同分子形式模式表明，前体在不同群体的内分泌细胞和神经元中通过不同途径进行加工。
