Herpers M J, Budka H, McCormick D
Acta Neuropathol. 1984;64(4):333-8. doi: 10.1007/BF00690398.
In 80 specimens of human glioma the production of glial fibrillary acidic protein (GFAP) by tumour cells invading meninges or connective tissue was studied immuno-cytochemically by the PAP technique. In 38 of 55 cases of astrocytoma, glioblastoma, gliosarcoma, and oligoastrocytoma, GFAP immunoreactivity was greater in the invading cells as compared with the main part of the neoplasm. Fifty-eight percent of the astroglial tumours invading the leptomeninges, all astroglial tumours invading connective tissue and all gliosarcomas showed enhanced GFAP immuno-reactivity of tumour cells getting in contact with collagenous tissue, whereas meningeal infiltrates of 25 non-astroglial tumours (oligodendroglioma, ependymoma, medulloblastoma) remained GFAP-negative like the main part of the respective tumours. In the majority of astroglial tumours an increase of GFAP immunoreactivity was found also in perivascular cells of the main part of the tumour. It is concluded that glioma cells are capable of adapting their cytoskeleton to their micro-environment. Contact with dense collagenous tissue appears as an important factor able to induce an increased production of GFAP by adjacent glial cells.
采用PAP技术，通过免疫细胞化学方法研究了80例人脑胶质瘤中侵袭脑膜或结缔组织的肿瘤细胞产生胶质纤维酸性蛋白（GFAP）的情况。在55例星形细胞瘤、胶质母细胞瘤、胶质肉瘤和少突星形细胞瘤中，有38例的侵袭细胞中GFAP免疫反应性比肿瘤主体部分更强。侵袭软脑膜的星形胶质细胞瘤中有58%、所有侵袭结缔组织的星形胶质细胞瘤以及所有胶质肉瘤中，与胶原组织接触的肿瘤细胞的GFAP免疫反应性增强，而25例非星形胶质细胞瘤（少突胶质细胞瘤、室管膜瘤、髓母细胞瘤）的脑膜浸润与各自肿瘤主体部分一样，GFAP呈阴性。在大多数星形胶质细胞瘤中，肿瘤主体部分的血管周围细胞中也发现GFAP免疫反应性增加。结论是，胶质瘤细胞能够使其细胞骨架适应其微环境。与致密胶原组织接触似乎是能够诱导相邻胶质细胞增加GFAP产生的一个重要因素。
