大肠杆菌中6-磷酸葡萄糖酸脱氢酶定向进化过程中发生的特异性缺失。
Specific deletion occurring in the directed evolution of 6-phosphogluconate dehydrogenase in Escherichia coli.
作者信息
Miller R D, Dykhuizen D E, Green L, Hartl D L
出版信息
Genetics. 1984 Dec;108(4):765-72. doi: 10.1093/genetics/108.4.765.
Abstract
A novel genetic change leading to increased activity of 6-phosphogluconate dehydrogenase (6PGD) in E. coli has been observed. The mutation is a deletion of approximately 0.4 kilobase pairs occurring between the structural gene of 6PGD (gnd) and one copy of an insertion element (IS5) found normally in E. coli K12 a few hundred base pairs upstream (counterclockwise) from gnd at 44 minutes on the conventional genetic map. The deletion is associated with a threefold higher activity of 6PGD and a 57% increase in the maximum growth rate when cells are grown in gluconate.
摘要
在大肠杆菌中观察到一种导致6-磷酸葡萄糖酸脱氢酶（6PGD）活性增加的新型基因变化。该突变是在6PGD（gnd）结构基因与通常在大肠杆菌K12中发现的一个插入元件（IS5）拷贝之间发生的约0.4千碱基对的缺失，该插入元件在传统遗传图谱上位于gnd上游（逆时针方向）几百个碱基对处，在44分钟位置。当细胞在葡萄糖酸盐中生长时，这种缺失与6PGD活性高三倍以及最大生长速率提高57%相关。
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