Yoshida Y, Nakano Y, Yamashita Y, Koga T
Department of Preventive Dentistry, Kyushu University Faculty of Dentistry, Maidashi, Higashi-ku, Japan.
Biochem Biophys Res Commun. 1997 Jan 3;230(1):220-5. doi: 10.1006/bbrc.1996.5917.
A 10-kb DNA fragment containing the gnd gene from Actinobacillus actinomy-cetemcomitans Y4 was isolated and sequenced. The structural gnd gene codes for 6-phosphogluconate dehydrogenase that consists of 484 amino acids. In contrast to the gnd gene in Escherichia coli, Salmonella typhimurium, or Klebsiella pneumoniae, the gnd gene of A. actinomycetemcomitans was not located in the rfb or cps operon. The zwf gene encoding glucose 6-phosphate dehydrogenase, which is another enzyme consisting of pentose-phosphate pathway, sided at 3.8-kb upstream from the gnd gene. A phylogenetic tree based on sequence analyses showed higher homology of 6-phospho-gluconate dehydrogenase of A. actinomycetemcomitans with the eucaryotic enzymes rather than with bacterial enzymes.
从伴放线放线杆菌Y4中分离出一个包含gnd基因的10kb DNA片段并进行测序。结构gnd基因编码由484个氨基酸组成的6-磷酸葡萄糖酸脱氢酶。与大肠杆菌、鼠伤寒沙门氏菌或肺炎克雷伯菌中的gnd基因不同，伴放线放线杆菌的gnd基因不在rfb或cps操纵子中。编码6-磷酸葡萄糖脱氢酶的zwf基因，这是戊糖磷酸途径中的另一种酶，位于gnd基因上游3.8kb处。基于序列分析的系统发育树显示，伴放线放线杆菌的6-磷酸葡萄糖酸脱氢酶与真核酶的同源性高于与细菌酶的同源性。
