糖皮质激素对淋巴因子诱导的巨细胞形成的调节作用。

Glucocorticoid modulation of lymphokine-induced giant cell formation.

作者信息

Galindo B

出版信息

Inflammation. 1984 Dec;8(4):393-406. doi: 10.1007/BF00918215.

Abstract

Lymph node lymphocytes from rabbits sensitized with bacillus Calmet Guerin (BCG) secreted into the culture media both macrophage fusion factor (MFF) and migration inhibition factor (MIF) after 24 h of incubation with heat-killed BCG. Cell-free supernatant fluids obtained from these cultures induced simultaneously giant cell formation and migration inhibition of homologous normal alveolar macrophages. The glucocorticoids cortisol (10(-7) M) and dexamethasone (10(-8) M) (DX) consistently inhibited giant cell formation elicited by MFF (P = 0.003) without affecting macrophage viability. By contrast, the same glucocorticoids, in concentrations ranging from 10(-8) to 10(-10) M, induced a considerable increment of giant cell development in macrophage populations exhibiting a low response to MFF. Neither cortisol (10(-4) M) nor DX (10(-4) M) affected the migration inhibition of alveolar macrophages induced by MIF. Present results suggest that the granulomatous response in the rabbit, as reflected by the macrophage fusion assay, may be regulated by glucocorticoids.

摘要

用卡介苗（BCG）致敏的家兔淋巴结淋巴细胞在与热灭活的BCG孵育24小时后，会将巨噬细胞融合因子（MFF）和迁移抑制因子（MIF）分泌到培养基中。从这些培养物中获得的无细胞上清液能同时诱导同源正常肺泡巨噬细胞形成巨细胞并抑制其迁移。糖皮质激素皮质醇（10⁻⁷ M）和地塞米松（10⁻⁸ M）（DX）持续抑制MFF引发的巨细胞形成（P = 0.003），且不影响巨噬细胞活力。相比之下，相同浓度范围为10⁻⁸至10⁻¹⁰ M的糖皮质激素，在对MFF反应较低的巨噬细胞群体中，会诱导巨细胞发育显著增加。皮质醇（10⁻⁴ M）和DX（10⁻⁴ M）均不影响MIF诱导的肺泡巨噬细胞迁移抑制。目前的结果表明，巨噬细胞融合试验所反映的家兔肉芽肿反应可能受糖皮质激素调节。