Hollings P E, Fitzgerald P H, Heaton D C, Beard M E
Int J Cell Cloning. 1984 Nov;2(6):348-55. doi: 10.1002/stem.5530020603.
Two female patients who received bone marrow transplants from male donors showed the donor karyotype in all blood cells cultured with phytohemagglutinin and in all cells of bone marrow aspirate after direct or 24-hour culture preparation for cytogenetic study. The donor origin of myeloid cells was demonstrated specifically by identifying the male karyotype in the dividing cells of granulocyte/macrophage colonies in soft agar. Only the host female karyotype was found in fibroblast cell populations from cultured bone marrow aspirate. Bone marrow aspirate from transplant patients contained cells of host origin and cells of donor origin; both will proliferate in culture.
两名接受男性供体骨髓移植的女性患者，在经植物血凝素培养的所有血细胞以及用于细胞遗传学研究的直接制备或24小时培养制备后的骨髓穿刺液的所有细胞中，均显示出供体核型。通过在软琼脂中粒细胞/巨噬细胞集落的分裂细胞中鉴定出男性核型，特异性地证明了髓细胞的供体来源。在培养的骨髓穿刺液的成纤维细胞群体中仅发现宿主女性核型。移植患者的骨髓穿刺液中含有宿主来源的细胞和供体来源的细胞；两者在培养中均会增殖。
