Ivanov-Smolenskiĭ A A, Gorskaia Iu F, Kuralesova A I, Latsinik N V
Biull Eksp Biol Med. 1976 Oct;82(10):1270-1.
The method of indirect immunofluorescence with the use of the isolinear antiserum was applied to the study of the linear reference of fibroblasts growing in the monolayer bone marrow cultures from the semisyngenous heterotropic transplants and radiochimerae. As shown, fibroblasts from the bone marrow of complete radiochimerae were of the recipient origin, whereas the histiocytes-macrophages (in the same cultures) - of donor origin. All the fibroblasts in the bone marrow cultures of the heterotropic transplant belonged to the initial donor, whereas 80% of the histiocytes were recipent's. This is a direct proof of the fact that stromal mechanocytes and hemopoietic cells, and also macrophages belonged to different cell lins.
采用间接免疫荧光法,利用等线性抗血清,对来自半同基因异向移植和放射嵌合体的单层骨髓培养物中生长的成纤维细胞的线性参考进行研究。结果表明,完全放射嵌合体骨髓中的成纤维细胞来源于受体,而组织细胞 - 巨噬细胞(在相同培养物中)来源于供体。异向移植骨髓培养物中的所有成纤维细胞均属于初始供体,而80%的组织细胞属于受体。这直接证明了基质机械细胞、造血细胞以及巨噬细胞属于不同细胞系这一事实。