Petts D N
J Appl Bacteriol. 1984 Dec;57(3):479-83. doi: 10.1111/j.1365-2672.1984.tb01413.x.
An improved selective broth, colistin, oxolinic acid, Todd Hewitt broth (COTHB) for the isolation of Streptococcus pyogenes and other streptococci from swabs is described. Staphylococci, coryneforms and Gram negative organisms are inhibited in COTHB inoculated with swabs from skin lesions. Streptococcus pyogenes could be detected by a fluorescent antibody method and by the Streptex and Phadebact streptococcal grouping methods after 6 and 24 h incubation. Other streptococci of groups B, C and G, were also detected. Streptex was the most sensitive and specific method for the detection of streptococci of groups A, B, C, and G in this broth. The method detected 78% of these streptococci in COTHB after 6 h incubation and 93% after 24 h incubation. Of the Strep. pyogenes isolated, 82% were detected in the broth by the Streptex method.
本文描述了一种改良的选择性肉汤——黏菌素、恶喹酸、托德-休伊特肉汤（COTHB），用于从拭子中分离化脓性链球菌和其他链球菌。用皮肤损伤拭子接种COTHB后，葡萄球菌、棒状杆菌和革兰氏阴性菌受到抑制。经6小时和24小时培养后，可通过荧光抗体法以及链球菌分类试剂（Streptex）和法德巴克（Phadebact）链球菌分组法检测到化脓性链球菌。还检测到了B、C和G组的其他链球菌。在这种肉汤中，链球菌分类试剂是检测A、B、C和G组链球菌最灵敏、特异的方法。该方法在COTHB中经6小时培养后能检测到78%的这些链球菌，24小时培养后能检测到93%。在分离出的化脓性链球菌中，82%可通过链球菌分类试剂法在肉汤中检测到。
