[Detection of antibodies against D-penicillamine. 2. Experimental animal proof of production of antibodies against D-penicillamine using indirect immunofluorescence with a DASS system].

Antibodies against D-penicillamine conjugates produced in animals be proved by indirect immunofluorescence by means of the DASS system (defined antigen substrate spheres). The artificial substrate (Sephadex G-25) has not been bound to the D-penicillamine conjugates used for immunization, but only to D-penicillamine, which justifies the assumption that antibodies against D-penicillamine could be registered and proved by the immunofluorescence test mentioned.