Breitenecker G, Friedrich F, Kemeter P
Fertil Steril. 1978 Mar;29(3):336-41.
Human ovarian follicles showed activity of alkaline phosphatase, acid phosphatase (AcP) leucine aminopeptidase, and lactate dehydrogenase (LDH) in the theca interna. The granulosa of nonovulatory tertiary follicles showed moderate activity, whereas that of preovulatory Graafian follicles showed strong activity of LDH and AcP. The activity of these enzymes in the follicular fluid was measured. In nonovulatory tertiary follicles, activity of 3beta-ol-steroid dehydrogenase (3beta-OHSD) was found only in the theca interna; their fluid contained 290 ng/ml of progesterone and 502 ng/ml of 17beta-estradiol (average). Preovulatory Graafian follicles showed activity of 3beta-OHSD in the theca as well as in the granulosa. The progesterone concentration of the fluid was 7037 ng/ml and the 17beta-estradiol concentration was 2800 ng/ml (average). More oocytes could be aspirated from ovaries of younger women than from those of older women. In both age groups one out of three oocytes was degenerated. Oocytes with preovulatory changes were found only in follicles with preovulatory changes in their walls. Degenerated oocytes were found in some nonovulatory follicles as well as in some follicles with preovulatory changes in their walls.
人卵巢卵泡的卵泡内膜显示出碱性磷酸酶、酸性磷酸酶（AcP）、亮氨酸氨肽酶和乳酸脱氢酶（LDH）的活性。未排卵的三级卵泡的颗粒细胞显示出中等活性，而排卵前的格拉夫卵泡的颗粒细胞显示出较强的LDH和AcP活性。对卵泡液中这些酶的活性进行了测定。在未排卵的三级卵泡中，3β-羟类固醇脱氢酶（3β-OHSD）的活性仅在卵泡内膜中发现；其卵泡液中平均含有290 ng/ml的孕酮和502 ng/ml的17β-雌二醇。排卵前的格拉夫卵泡在卵泡内膜和颗粒细胞中均显示出3β-OHSD的活性。卵泡液中孕酮浓度平均为7037 ng/ml，17β-雌二醇浓度平均为2800 ng/ml。年轻女性卵巢中吸出的卵母细胞比老年女性的多。在两个年龄组中，三分之一的卵母细胞发生退化。仅在壁有排卵前变化的卵泡中发现有排卵前变化的卵母细胞。在一些未排卵的卵泡以及壁有排卵前变化的一些卵泡中发现有退化的卵母细胞。
