Carson R S, Trounson A O, Findlay J K
J Clin Endocrinol Metab. 1982 Oct;55(4):798-800. doi: 10.1210/jcem-55-4-798.
Oocytes and matched samples of follicular fluid were obtained from 156 pre-ovulatory follicles in 125 women 26--36 h after either administration of hCG or the onset of an endogenous LR surge. Concentrations of estradiol-17 beta (E2), progesterone (P) and androstenedione (A4) in the fluid of individual donor follicles were measured and related to the success of fertilisation of oocytes in vitro and the incidence of pregnancies after embryo transfer. Oocytes which gave rise to successful pregnancies were obtained from follicles which contained greater concentrations of E2 and a higher ratio of E2:P than did oocytes from which pregnancy did not result. These data provide direct evidence in support of the hypothesis that estrogenic follicles are the sole source of ova which undergo fertilisation and subsequently give rise to pregnancy in women.
在125名女性中，于注射人绒毛膜促性腺激素（hCG）后或内源性促黄体生成素（LH）高峰出现后26 - 36小时，从156个排卵前卵泡中获取卵母细胞及配对的卵泡液样本。测定各个供体卵泡液中雌二醇-17β（E2）、孕酮（P）和雄烯二酮（A4）的浓度，并将其与卵母细胞体外受精的成功率以及胚胎移植后的妊娠发生率相关联。成功妊娠的卵母细胞来自于E2浓度更高且E2:P比值高于未妊娠卵母细胞的卵泡。这些数据提供了直接证据，支持了雌激素化卵泡是女性中经历受精并随后导致妊娠的卵子唯一来源这一假说。
