Channing C P, Liu C Q, Jones G S, Jones H
Proc Natl Acad Sci U S A. 1983 Jul;80(13):4184-8. doi: 10.1073/pnas.80.13.4184.
To examine whether a decline in follicular oocyte maturation inhibitor (OMI) is associated with attainment of oocyte maturation and fertilizability, OMI was measured in follicular fluid (FF) of 39 follicles of 20 normal women given human menopausal gonadotrophin and human chorionic gonadotrophin to induce follicular growth and maturation. Oocytes were aspirated per laparoscope, the fluid was saved, and the egg was observed, incubated, and inseminated with the husband's sperm. Concepti that developed to the 4- to 8-cell stage were transferred to the uterus and the women were followed for pregnancy. OMI activity in each FF was measured by using cultured cumulus-enclosed porcine oocytes (30-40 oocytes per FF sample). Estrogen, progesterone, oocytes (30-40 oocytes per FF sample). Estrogen, progesterone, and delta 4-androstenedione were measured in FF by radioimmunoassay. The FF of 13 preovulatory follicles yielding oocytes that were mature and fertilizable had significantly less OMI activity (mean +/- SEM) (0.58 +/- 0.10 unit/ml) compared to follicles yielding immature oocytes (2.8 +/- 0.56 units/ml; n = 9), atretic oocytes (5.5 +/- 2.5 units/ml; n = 7), or preovulatory oocytes with fractured zonae (1.9 +/- 0.63 units/ml; n = 7). The estrogen concentration (mean +/- SEM) of preovulatory follicles yielding mature fertilizable eggs or mature eggs with fractured zonae was greater (396 +/- 34 ng/ml; n = 20) compared to follicles yielding immature or atretic eggs (203 +/- 59 ng/ml; n = 9 and 97 +/- 47 ng/ml; n = 7, respectively; P less than 0.05). Progesterone concentration (mean +/- SEM; ng/ml) of FF was generally elevated in all preovulatory follicles (635 +/- 53) compared to immature or atretic follicles (230 +/- 64 and 76 +/- 17, respectively; P less than 0.05). It may be concluded that in normal follicle maturation there is a decline in OMI in the follicle containing an oocyte that becomes mature and fertilizable. There is also an increase in estrogen, progesterone, and follicle size. It is also possible to have an abnormal follicle maturation when there is an increase in size as well as FF, estrogen, and progesterone, but withut a decline in OMI--a situation which can lead to production of a nonfertilizable oocyte.
为了研究卵泡卵母细胞成熟抑制因子（OMI）水平的下降是否与卵母细胞成熟及受精能力的获得有关，对20名正常女性的39个卵泡的卵泡液（FF）中的OMI进行了检测。这些女性接受了人绝经期促性腺激素和人绒毛膜促性腺激素以诱导卵泡生长和成熟。通过腹腔镜吸取卵母细胞，保存卵泡液，并观察、培养卵子，然后用丈夫的精子进行授精。将发育到4至8细胞阶段的胚胎移植到子宫内，并对这些女性进行妊娠随访。每个卵泡液中的OMI活性通过培养的卵丘包裹的猪卵母细胞（每个卵泡液样本30 - 40个卵母细胞）进行测量。通过放射免疫分析法测定卵泡液中的雌激素、孕酮和δ4 - 雄烯二酮。与产生未成熟卵母细胞的卵泡（2.8±0.56单位/毫升；n = 9）、闭锁卵母细胞的卵泡（5.5±2.5单位/毫升；n = 7）或卵透明带破裂的排卵前卵母细胞的卵泡（1.9±0.63单位/毫升；n = 7）相比，13个产生成熟且可受精的卵母细胞的排卵前卵泡的卵泡液中OMI活性显著降低（平均值±标准误）（0.58±0.10单位/毫升）。与产生未成熟或闭锁卵母细胞的卵泡相比（分别为203±59纳克/毫升；n = 9和97±47纳克/毫升；n = 7），产生成熟可受精卵或卵透明带破裂的成熟卵的排卵前卵泡的雌激素浓度（平均值±标准误）更高（396±34纳克/毫升；n = 20）（P＜0.05）。与未成熟或闭锁卵泡相比（分别为230±64和76±17），所有排卵前卵泡的卵泡液中孕酮浓度（平均值±标准误；纳克/毫升）普遍升高（635±53）（P＜0.05）。可以得出结论，在正常卵泡成熟过程中，含有成熟且可受精卵母细胞的卵泡中OMI水平下降。雌激素、孕酮和卵泡大小也会增加。当卵泡大小以及卵泡液、雌激素和孕酮增加，但OMI水平未下降时，也可能出现异常卵泡成熟——这种情况可能导致产生不可受精的卵母细胞。
