Zanetta J P, Roussel G, Dontenwill M, Vincendon G
J Neurochem. 1983 Jan;40(1):202-8. doi: 10.1111/j.1471-4159.1983.tb12671.x.
The localization of alpha-D-mannosidase in the rat cerebellum was studied by using indirect immunohistochemistry at both optical and electron microscopic levels. In the adult the enzyme is particularly concentrated in the dendrites and cell bodies of Purkinje cells, basket cells, and Golgi neurons in the cerebellar cortex and in the cytoplasm and dendrites of deep nuclei neurons. The cytoplasm of granule cells is poorly stained, whereas parallel fibers, white matter, Bergman fibers, and Golgi epitheloid cell perikarya show virtually no staining. Electron microscopy suggests that most of the staining is found in the cytosol, although some staining is found in the postsynaptic densities of the synapses between parallel fibers and Purkinje dendrites. The pattern of staining was followed throughout the postnatal development of the rat cerebellum. At bith an intense and diffuse staining is found in all cells except those of the external germinative layer. At the 6th postnatal day, Purkinje cell bodies and apical cones are strongly labeled. From the 13th day on the pattern is very similar to that found in the adult. However, at the 18th postnatal day (when compared with the other structures), the staining of Purkinje cell dendrites seems to be higher than at all other ages. These data are correlated with biochemical studies and discussed in relation to the possible role of this enzyme during the postnatal development of the rat cerebellum.
采用间接免疫组织化学方法，在光学显微镜和电子显微镜水平上研究了大鼠小脑α-D-甘露糖苷酶的定位。在成年大鼠中，该酶特别集中在小脑皮质浦肯野细胞、篮状细胞和高尔基神经元的树突及细胞体中，以及深部核团神经元的细胞质和树突中。颗粒细胞的细胞质染色较浅，而平行纤维、白质、伯格曼纤维和高尔基上皮样细胞的胞体几乎没有染色。电子显微镜观察表明，大部分染色位于胞质溶胶中，尽管在平行纤维与浦肯野树突之间突触的突触后致密物中也发现了一些染色。在大鼠小脑出生后的整个发育过程中都对染色模式进行了跟踪。出生时，除了外生发层细胞外，所有细胞都有强烈而弥漫的染色。出生后第6天，浦肯野细胞体和顶锥被强烈标记。从第13天开始，染色模式与成年大鼠非常相似。然而，在出生后第18天（与其他结构相比），浦肯野细胞树突的染色似乎比其他所有年龄段都高。这些数据与生化研究相关，并就该酶在大鼠小脑出生后发育过程中的可能作用进行了讨论。
