Brüning G
Department of Anatomy, Free University of Berlin, Germany.
J Neurosci Res. 1993 Dec 1;36(5):580-7. doi: 10.1002/jnr.490360510.
NADPH-diaphorase histochemistry has been applied for the localization of nitric oxide synthase during the postnatal development of the mouse cerebellum. Staining for NADPH-diaphorase during the first week after birth was confined to some but not all laminae of the immature cerebellum: NADPH-diaphorase activity was located in the molecular/Purkinje cell layer and in inner parts of the internal granular layer. The external granular layer and the developing white matter were essentially unstained. Expression was earliest and very strong in parallel fibers and in the internal granule layer of the ventral part of the pyramis and the dorsal part of the uvula. Staining in the Purkinje cell layer was observed throughout the cerebellum. The presence of formazan deposits within Purkinje cells was verified by colocalization with calbindin D-28k immunoreactivity. The distribution of NADPH-diaphorase activity changed into the adult pattern between 8 and 12 days of age: Within the molecular layer, basket cells and their processes became strongly stained. Reaction product within Purkinje cells gradually disappeared. Likewise, strongly stained parallel fibers were no longer detectable. These results suggest that nitric oxide is involved in different processes in cerebellar development.
NADPH黄递酶组织化学已被应用于小鼠小脑出生后发育过程中一氧化氮合酶的定位研究。出生后第一周，NADPH黄递酶染色局限于未成熟小脑的部分而非所有层：NADPH黄递酶活性位于分子层/浦肯野细胞层以及内颗粒层内部。外颗粒层和发育中的白质基本未染色。在平行纤维以及蚓锥体腹侧和蚓垂背侧的内颗粒层中，表达最早且非常强烈。在整个小脑中均观察到浦肯野细胞层有染色。通过与钙结合蛋白D - 28k免疫反应性共定位，证实了浦肯野细胞内有甲臜沉淀。在8至12日龄之间，NADPH黄递酶活性的分布转变为成年模式：在分子层内，篮状细胞及其突起被强烈染色。浦肯野细胞内的反应产物逐渐消失。同样，不再能检测到强烈染色的平行纤维。这些结果表明一氧化氮参与了小脑发育的不同过程。
