Rizki T M, Rizki R M
Science. 1983 Apr 1;220(4592):73-5. doi: 10.1126/science.6402819.
When wheat germ agglutinin conjugated to fluorescein isothiocyanate is bound to hemocytes from larvae of Drosophila melanogaster, two populations of hemocytes are distinguished. One shows a fluorescent speckled surface (spk+) and the other lacks this characteristic (spk-). In mutant larvae with melanotic tumors and in larval hosts with heterospecific implants, most of the lamellocytes (a hemocyte variant involved in capsule formation and tissue rejection) are spk+, whereas the lamellocytes in nontumorous larvae are spk-. This suggests that spk+ lamellocytes are necessary for encapsulation of aberrant tissues in the mutant larvae and are responsible for rejection of foreign tissue implants.
当与异硫氰酸荧光素结合的麦胚凝集素与黑腹果蝇幼虫的血细胞结合时，可区分出两类血细胞。一类呈现荧光斑点状表面（spk+），另一类则缺乏这一特征（spk-）。在患有黑素瘤的突变幼虫以及带有异种植入物的幼虫宿主中，大多数片状细胞（一种参与包囊形成和组织排斥的血细胞变体）是spk+，而在无肿瘤的幼虫中，片状细胞是spk-。这表明spk+片状细胞对于突变幼虫中异常组织的包囊形成是必需的，并且负责对外来组织植入物的排斥。
