Denys G A, Newman M A, Standard P G
Am J Clin Pathol. 1983 Mar;79(3):379-81. doi: 10.1093/ajcp/79.3.379.
Seventy-nine mycelial-form stock cultures of Blastomyces dermatitidis, Coccidioides immitis, Histoplasma capsulatum, and morphologically similar fungi were extracted and tested by using commercial macroimmunodiffusion exoantigen test kits and the Centers for Disease Control (CDC) reference system for identifying fungal isolates. Results showed 100% correlation between the two systems. Specific exoantigens of C. immitis and H. capsulatum extracted from agar slant cultures (slant extraction method) readily were identified. In eight of 26 cultures of B. dermatitidis, broth culture filtrates (broth-shake-flask method) were required to demonstrate the specific bands of identity. No false-negative reactions or cross-reactivity among the pathogens and other fungi were observed. The commercial test kits provided a rapid and specific method for identifying or confirming suspected fungal pathogens.
提取了79株皮炎芽生菌、粗球孢子菌、荚膜组织胞浆菌以及形态学相似真菌的丝状菌形式的菌种培养物，使用商用宏观免疫扩散外抗原检测试剂盒和疾病控制中心（CDC）参考系统对其进行检测，以鉴定真菌分离株。结果显示这两种系统之间的相关性为100%。从琼脂斜面培养物中提取的粗球孢子菌和荚膜组织胞浆菌的特异性外抗原（斜面提取法）很容易被鉴定出来。在26株皮炎芽生菌培养物中的8株中，需要肉汤培养滤液（肉汤摇瓶法）来显示特异性的鉴定条带。未观察到病原体与其他真菌之间的假阴性反应或交叉反应。商用检测试剂盒为鉴定或确认疑似真菌病原体提供了一种快速且特异的方法。
