Phillips M W, Lee A
Appl Environ Microbiol. 1983 Feb;45(2):675-83. doi: 10.1128/aem.45.2.675-683.1983.
Spiral-shaped bacteria with a distinctive morphology were isolated from the intestinal mucosa of rats and mice on a campylobacter selective medium using microaerophilic incubation. These bacteria have been shown by other authors to be present in the intestinal tracts of several animal species but have not been cultured previously. The results of electron microscopic examinations and biochemical testing have shown that these organisms do not correspond to any known genus. Colonization experiments with pure cultures in gnotobiotic rodents have shown these bacteria to be mucosa associated, with a particular affinity for intestinal crypts. The pattern of colonization of the intestinal crypts in gnotobiotes known to be free of other mucosa-associated organisms differed from the colonization occurring in conventional animals that possess a normal mucosa-associated flora.
在弯曲杆菌选择性培养基上，采用微需氧培养法，从大鼠和小鼠的肠黏膜中分离出具有独特形态的螺旋形细菌。其他作者已表明这些细菌存在于多种动物的肠道中，但此前尚未进行培养。电子显微镜检查和生化测试结果表明，这些微生物与任何已知属均不相符。在无菌啮齿动物中进行的纯培养物定殖实验表明，这些细菌与黏膜相关，对肠隐窝具有特殊亲和力。已知不含其他黏膜相关微生物的无菌动物中肠隐窝的定殖模式与具有正常黏膜相关菌群的常规动物中发生的定殖模式不同。
