喂食和禁食大鼠分离肝细胞中ATP、ADP和PO4的分配情况

Repartition of ATP, ADP and PO4 in isolated hepatocytes from fed and fasted rats.

Lavoinne A

Biochimie. 1983 Jan;65(1):71-5. doi: 10.1016/s0300-9084(83)80031-5.

Abstract

The digitonin fractionation procedure [Zuurendonk, P. F. and Tager, J. M. (1974) Biochim. Biophys. Acta, 333, 393-399] was used to determine the repartition of adenine nucleotides and inorganic phosphate in isolated hepatocytes from fed and fasted rats. 2. This repartition is not significantly modified in the presence of pyruvate or alanine or lactate + pyruvate for isolated hepatocytes from fasted rats. 3. In isolated hepatocytes from fasted rats, the mitochondrial ATP/ADP X PO4 ratio is two-fold lower than in isolated hepatocytes from fed rats. 4. The cytosolic ATP/ADP X PO4 ratio depends on the nutritional state and (or) on the added substrate for neoglucogenesis.

摘要

采用洋地黄皂苷分级分离法[祖伦登克，P. F. 和塔格，J. M.（1974年）《生物化学与生物物理学报》，333卷，393 - 399页]来测定喂食和禁食大鼠分离肝细胞中腺嘌呤核苷酸和无机磷酸盐的重新分配情况。2. 对于禁食大鼠的分离肝细胞，在丙酮酸、丙氨酸或乳酸 + 丙酮酸存在的情况下，这种重新分配没有显著改变。3. 在禁食大鼠的分离肝细胞中，线粒体ATP/ADP×PO4比值比喂食大鼠的分离肝细胞低两倍。4. 胞质ATP/ADP×PO4比值取决于营养状态和（或）新糖生成所添加的底物。