乙硫氨酸对分离的肝细胞中腺嘌呤核苷酸磷酸化状态的影响。

The influence of ethionine on the phosphorylation state of adenine nucleotides in isolated hepatocytes.

作者信息

Lavoinne A, Marchand J C, Pinosa M, Matray F

出版信息

Biochimie. 1983 Aug-Sep;65(8-9):471-6. doi: 10.1016/s0300-9084(83)80128-x.

Abstract

The influence of D,L-ethionine (5 mM) was tested on hepatocytes isolated from fed or fasted rats. The digitonine fractionation procedure [6] was used to determine the repartition of adenine nucleotides and inorganic phosphate. Ethionine induces a decrease in the intracellular ATP content; this decrease is located both in the cytosol and in the mitochondria of fed and fasted rat hepatocytes. The observed effects of ethionine are: 1) fed rats: increase in glycolysis, decrease in mitochondrial ATP/ADP x PO4 and mitochondrial NAD+/NADH ratios; 2) fasted rats: decrease in neoglucogenesis from lactate + pyruvate or alanine, decrease in cytosolic ATP/ADP x PO4 and cytosolic NAD/NADH ratios.

摘要

测试了D，L-乙硫氨酸（5 mM）对从喂食或禁食大鼠分离的肝细胞的影响。采用洋地黄皂苷分级分离法[6]来确定腺嘌呤核苷酸和无机磷酸盐的重新分配。乙硫氨酸会导致细胞内ATP含量降低；这种降低在喂食和禁食大鼠肝细胞的细胞质和线粒体中均有发生。观察到的乙硫氨酸的作用为：1）喂食大鼠：糖酵解增加，线粒体ATP/ADP×PO4以及线粒体NAD+/NADH比值降低；2）禁食大鼠：乳酸+丙酮酸或丙氨酸的糖异生减少，细胞质ATP/ADP×PO4以及细胞质NAD/NADH比值降低。