Adejumo Isaac Oluseun
Department of Animal Science, University of Ibadan, Ibadan, Nigeria.
Genes Nutr. 2024 Oct 19;19(1):21. doi: 10.1186/s12263-024-00755-4.
Lactic acid bacteria (LAB) probiotics are health-promoting but their characteristics, safety profile and functional mechanisms are not fully understood. Hence, this study aimed to characterize some hypothetical proteins of the chicken-isolated Limosilactobacillus reuteri genome and unravel their IL-2 and IL-10-inducing potential to understand mechanisms of their immunological functionality for sustainable applications. The selected proteins were subjected to in silico analyses for transmembrane topology, sub-cellular localization, IL-2 and IL-10-inducing ability and IL-2 and IL-10 gene expression across various conditions. IL-2 and IL-10-inducing mutants were statistically analyzed using a one-way analysis of variance of a general linear model of SAS and statistical significance was set at p < 0.05. The analyzed proteins are stable under a wide temperature range. All the hypothetical proteins are IL-2 and IL-10-inducing but QHPv.2.12, QHPv.2.13 and QHPv.2.15 are non-immunogenic. The evaluated mutants are IL-2 and IL-10-inducers but QHPv.2.13 and QHPv.2.15 are not immunogenic. This study sheds light on understanding the functional mechanisms of chicken-isolated L. reuteri and suggests it or its proteins as potent candidates for feed additive and therapeutic purposes.
乳酸菌（LAB）益生菌对健康有益，但其特性、安全性和功能机制尚未完全明确。因此，本研究旨在对从鸡体内分离出的罗伊氏乳杆菌基因组中的一些假定蛋白质进行表征，并揭示它们诱导白细胞介素-2（IL-2）和白细胞介素-10（IL-10）的潜力，以了解其免疫功能机制，从而实现可持续应用。对所选蛋白质进行了计算机模拟分析，以确定其跨膜拓扑结构、亚细胞定位、诱导IL-2和IL-10的能力以及在各种条件下IL-2和IL-10基因的表达情况。使用SAS通用线性模型的单因素方差分析对诱导IL-2和IL-10的突变体进行统计分析，设定统计学显著性为p < 0.05。分析的蛋白质在较宽的温度范围内稳定。所有假定蛋白质均能诱导IL-2和IL-10，但QHPv.2.12、QHPv.2.13和QHPv.2.15无免疫原性。评估的突变体是IL-2和IL-10诱导剂，但QHPv.2.13和QHPv.2.15无免疫原性。本研究有助于理解从鸡体内分离出的罗伊氏乳杆菌的功能机制，并表明其或其蛋白质有望成为饲料添加剂和治疗用途的有力候选物。
