Agard D A, Sedat J W
Nature. 1983 Apr 21;302(5910):676-81. doi: 10.1038/302676a0.
The three-dimensional chromosome topography in an intact nucleus has been determined using fluorescently stained Drosophila polytene chromosomes, optical fluorescence microscopy and newly developed, generally applicable, cellular image reconstruction techniques. The folding pattern is a complex mixture of parallel chromosomal segments and intertwined coils and shows extensive interaction of the chromosomes with the nuclear envelope.
利用荧光染色的果蝇多线染色体、光学荧光显微镜以及新开发的、普遍适用的细胞图像重建技术，已确定了完整细胞核中的三维染色体拓扑结构。折叠模式是平行染色体片段和缠绕线圈的复杂混合，并且显示出染色体与核膜之间广泛的相互作用。
