Gruenbaum Y, Hochstrasser M, Mathog D, Saumweber H, Agard D A, Sedat J W
J Cell Sci Suppl. 1984;1:223-34. doi: 10.1242/jcs.1984.supplement_1.14.
The combination of optical fluorescence microscopy with digital image processing and analysis has been used to examine the three-dimensional organization of chromosomes within intact polytene nuclei. Although the arrangement indicates a high degree of flexibility, there are many conserved features between nuclei at the same developmental state. For example, chromosome arms are loosely coiled with centromeres clustered at the opposite end of the nucleus from the telomeres. Individual chromosome arms are not interwoven but occupy different spatial domains. Chromosomal sites that contact the envelope correlate with intercalary heterochromatin. Connections are observed between actively transcribing regions.
光学荧光显微镜与数字图像处理及分析相结合，已被用于研究完整多线核内染色体的三维组织。尽管这种排列显示出高度的灵活性，但处于相同发育状态的细胞核之间存在许多保守特征。例如，染色体臂松散地盘绕，着丝粒聚集在细胞核与端粒相对的一端。单个染色体臂并不相互交织，而是占据不同的空间域。与核膜接触的染色体位点与居间异染色质相关。在活跃转录区域之间观察到连接。
