Srikumaran S, Guidry A J, Goldsby R A
Science. 1983 Apr 29;220(4596):522-4. doi: 10.1126/science.6403985.
The interspecific fusion of normal bovine lymphocytes with a nonsecreting mouse hybridoma produced stable cell lines secreting bovine immunoglobulins. One of these lines has continued to secrete immunoglobulin G1 (5 to 10 micrograms per milliliter) for over 16 months. The bovine x mouse hybrid cells can be expected to provide bovine monoclonal immunoglobulins for sequencing studies and for use as serological standards as well as to provide messenger RNA for cloning bovine immunoglobulin genes.
正常牛淋巴细胞与非分泌型小鼠杂交瘤的种间融合产生了分泌牛免疫球蛋白的稳定细胞系。其中一个细胞系持续分泌免疫球蛋白G1（每毫升5至10微克）超过16个月。牛与小鼠的杂交细胞有望提供用于测序研究和用作血清学标准的牛单克隆免疫球蛋白，以及提供用于克隆牛免疫球蛋白基因的信使核糖核酸。
