Brown K, Gardner J J, Lockley W J, Preston J R, Wilkinson D J
Ann Clin Biochem. 1983 Jan;20 Pt 1:31-6. doi: 10.1177/000456328302000105.
A sensitive radioimmunoassay method for sodium cromoglycate in human plasma is described. The lowest quantifiable concentration of sodium cromoglycate is 0.93 nmol/l when 0.1 ml plasma samples are analysed. Direct analysis of sodium cromoglycate concentrations in plasma samples collected up to several hours after the administration of single therapeutic doses of the compound is possible. An antiserum raised in a sheep, radioligand heterogeneously labelled with iodine-125, and a second antibody technique for the separation of bound and free radioligand are employed. The inter-assay coefficient of variation is less than 14% (n = 23). The range of the method is limited; both 0.01 and 0.1 ml volumes of plasma must be analysed to encompass the concentration range 0.93-139 nmol/l which may be encountered in plasma samples from patients and human volunteers. The method is specific for sodium cromoglycate as indicated by a low cross-reactivity of the anti-cromoglycate antiserum with a number of drugs.
本文描述了一种用于测定人血浆中色甘酸钠的灵敏放射免疫分析方法。当分析0.1 ml血浆样品时，色甘酸钠的最低可定量浓度为0.93 nmol/l。对单次治疗剂量该化合物给药后数小时内采集的血浆样品中的色甘酸钠浓度进行直接分析是可行的。采用在绵羊体内产生的抗血清、用碘-125非均匀标记的放射性配体以及用于分离结合型和游离型放射性配体的第二抗体技术。批间变异系数小于14%（n = 23）。该方法的范围有限；必须分析0.01 ml和0.1 ml体积的血浆，以涵盖患者和人类志愿者血浆样品中可能出现的0.93 - 139 nmol/l的浓度范围。如抗色甘酸钠抗血清与多种药物的交叉反应性较低所示，该方法对色甘酸钠具有特异性。
