用于胃蛋白酶和其他羧基蛋白酶的光亲和试剂。

Photoaffinity reagents for use with pepsin and other carboxyl proteases.

作者信息

Hixson S H, Hurwitz J L, Langridge K J, Nichols D C, Provost K M, Wolff A M

出版信息

Biochem Biophys Res Commun. 1983 Mar 16;111(2):630-5. doi: 10.1016/0006-291x(83)90353-4.

Abstract

Two compounds have been designed to serve as photoaffinity reagents for use with carboxyl proteases. 1,2-Epoxy-3-(4'-azido-2'-nitrophenoxy)propane has been synthesized and shown to react with porcine pepsin in the same fashion as the traditional inhibitor 1,2-epoxy-3-(p-nitrophenoxy)propane, while p-azidophenacyl bromide is similar to other phenacyl bromides in its reaction with pepsin. In combination with p-azido-alpha-diazoacetophenone, previously shown to resemble alpha-diazo carbonyl reagents in its reaction with pepsin, photoaffinity analogs are now available for all three of the widely-used carboxyl protease inhibitors.

摘要

已设计出两种化合物作为用于羧基蛋白酶的光亲和试剂。1,2-环氧-3-(4'-叠氮基-2'-硝基苯氧基)丙烷已合成出来，并显示出与猪胃蛋白酶的反应方式与传统抑制剂1,2-环氧-3-(对硝基苯氧基)丙烷相同，而对叠氮苯甲酰溴在与胃蛋白酶的反应中与其他苯甲酰溴类似。与先前显示在与胃蛋白酶的反应中类似于α-重氮羰基试剂的对叠氮基-α-重氮苯乙酮相结合，现在对于所有三种广泛使用的羧基蛋白酶抑制剂都有光亲和类似物可用。