Vignon F, Derocq D, Chambon M, Rochefort H
C R Seances Acad Sci III. 1983 Jan 24;296(3):151-6.
In the MCF7 human breast cancer cells, estrogens stimulate in vitro cell proliferation and biosynthesis of specific proteins which are released into the culture medium; the most abundant of these is a 52,000 dalton glycoprotein. Conditioned media from E2-treated MCF7 cells stimulate the growth of E2-deprived MCF7 cells, whereas conditioned media from untreated control cells are ineffective. At protein concentration greater than 5 micrograms/ml, an inhibitory effect was also obtained with control media. The mitogenic factors of these conditioned media are non-dialysable, sensitive to temperature and proteases and adsorbed on Con A Sepharose. The cell surface of MCF7 cells, examined by scanning electron microscopy, is differentially affected by these two types of conditioned media. We conclude that estrogens may stimulate the growth of breast cancer cells indirectly via the induction of glycoproteins acting as growth factors.
在MCF7人乳腺癌细胞中，雌激素可刺激体外细胞增殖以及特定蛋白质的生物合成，这些蛋白质会释放到培养基中；其中最丰富的是一种52,000道尔顿的糖蛋白。来自经E2处理的MCF7细胞的条件培养基可刺激雌激素缺乏的MCF7细胞生长，而未经处理的对照细胞的条件培养基则无效。当蛋白质浓度大于5微克/毫升时，对照培养基也会产生抑制作用。这些条件培养基的促有丝分裂因子不可透析，对温度和蛋白酶敏感，并吸附在伴刀豆球蛋白A琼脂糖上。通过扫描电子显微镜检查发现，这两种类型的条件培养基对MCF7细胞的细胞表面有不同影响。我们得出结论，雌激素可能通过诱导作为生长因子的糖蛋白间接刺激乳腺癌细胞的生长。
