小牛血清对人淋巴母细胞源性干扰素产生的影响。
The effect of calf serum on human lymphoblastoid cell-derived interferon production.
作者信息
Klein F, Ricketts R T, Jones W I, Clark P M
出版信息
J Interferon Res. 1983;3(1):115-20. doi: 10.1089/jir.1983.3.115.
PMID:6404996
Abstract
Substitution of low-cost calf serum for fetal bovine serum was evaluated in Namalva cell production of human alpha-lymphoblastoid interferon [HuIFN-alpha(Ly)] in 50-liter fermentors. Namalva cell growth and IFN yields were greatly reduced and protein precipitate weight increased from calf serum-supplemented cell cultures. The overall reduction in IFN production was greater than threefold, making the substitution impractical.
摘要
在50升发酵罐中用人Namalva细胞生产人α-淋巴母细胞干扰素[HuIFN-α(Ly)]时，评估了用低成本小牛血清替代胎牛血清的效果。在添加小牛血清的细胞培养物中，Namalva细胞生长和干扰素产量大幅降低，蛋白质沉淀重量增加。干扰素产量总体降低超过三倍，使得这种替代不切实际。
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