Bock G R, Yates G K, Moorjani P A
J Neurosci Methods. 1983 Mar;7(3):227-33. doi: 10.1016/0165-0270(83)90004-3.
The Hewlett Packard Model 47210A Capnometer, intended for monitoring expired CO2 in human patients, can be modified for use in acute neurophysiological studies on small animals. The airway adaptor which would normally be attached to an endotracheal tube is modified and its internal volume is reduced to 0.2 ml, and is incorporated into a guinea pig head-holder. We have verified that the modification does not alter the Capnometer's accuracy. There is no significant increase in resistance in the airway when the adaptor is in place, and the waveform of instantaneous CO2 shows rapid changes on inspiration and expiration with well-resolved peaks and troughs. Measurement of arterial pCO2 suggests that the Capnometer and modified airway adaptor form an accurate system for obtaining a continuous record of end-tidal CO2 in small guinea pigs.
惠普47210A型二氧化碳监测仪旨在监测人类患者呼出的二氧化碳，经改装后可用于小动物的急性神经生理学研究。通常连接到气管插管上的气道适配器经过改装，其内部容积减小到0.2毫升，并被整合到豚鼠头部固定器中。我们已经证实，这种改装不会改变二氧化碳监测仪的准确性。当适配器就位时，气道阻力没有显著增加，瞬时二氧化碳波形在吸气和呼气时显示出快速变化，峰谷清晰可辨。动脉血二氧化碳分压的测量表明，二氧化碳监测仪和改装后的气道适配器构成了一个准确的系统，可用于连续记录小型豚鼠的呼气末二氧化碳。
