Ukponmwan O E, van der Poel-Heisterkamp A L, Haffmans J, Dzoljic M
Naunyn Schmiedebergs Arch Pharmacol. 1983 Feb;322(1):38-41. doi: 10.1007/BF00649350.
The relationship between deprenyl (MAO-B inhibitor), beta-phenylethylamine (PEA, MAO-B substrate) and [D-Ala2]-Met-enkephalinamide (DALA)-induced seizure was studied in the urethane-anaesthetized rats. A combined electromyographic (EMG) and electrocorticographic (ECoG) method was used. PEA (20-100 micrograms ivt) or DALA (10 micrograms ivt) induced myoclonic contractions (MC) in the submandibular muscle and epileptiform pattern with spike activity in the ECoG. Administration of subconvulsant doses of PEA (5-10 micrograms ivt 0.5-1 min before DALA) significantly increased DALA-induced seizure activity. Similarly, blockade of MAO-B with deprenyl (3-48 mg/kg ip) also enhanced DALA-induced epileptiform pattern. It is evident from this study that MAO-B system significantly modulates the excitatory phenomena induced by DALA. These findings of interactions between MAO-B system and enkephalinergic one, might be of relevance in the clinical situations such as psychosis, stress, a use of tricyclic antidepressants and all other cases, where the alteration of MAO-B system is a part of disease or induced during drug therapy.
在氨基甲酸乙酯麻醉的大鼠中研究了司来吉兰（单胺氧化酶B抑制剂）、β-苯乙胺（PEA，单胺氧化酶B底物）与[D-丙氨酸2]-甲硫氨酸脑啡肽酰胺（DALA）诱导的癫痫发作之间的关系。采用了肌电图（EMG）和皮质电图（ECoG）相结合的方法。PEA（静脉注射20 - 100微克）或DALA（静脉注射10微克）可诱导下颌下肌出现肌阵挛性收缩（MC），并在ECoG中出现伴有棘波活动的癫痫样模式。在DALA注射前0.5 - 1分钟静脉注射亚惊厥剂量的PEA（5 - 10微克）可显著增加DALA诱导的癫痫发作活动。同样，用司来吉兰（腹腔注射3 - 48毫克/千克）阻断单胺氧化酶B也可增强DALA诱导的癫痫样模式。从这项研究中可以明显看出，单胺氧化酶B系统显著调节DALA诱导的兴奋现象。单胺氧化酶B系统与脑啡肽能系统之间相互作用的这些发现，可能与精神病、应激、三环类抗抑郁药的使用以及所有其他单胺氧化酶B系统改变是疾病的一部分或在药物治疗过程中诱发的临床情况相关。
