Tejedor F, Ballesta J P
Anal Biochem. 1983 Jan;128(1):115-20. doi: 10.1016/0003-2697(83)90351-2.
A system for radioactive labeling of compounds of biological interest that, due to their low electronic density, cannot be labeled by the standard iodination techniques is described. Using p-nitroanisole as a model, we have prepared 2-[125I]iodo-4-nitroanisole by treatment with thallium trifluoroacetate, with later displacement of the thallium by iodide according to A. McKillop et al. (J. Amer. Chem. Soc. 93, 4841-4844 (1970)). The labeled iodonitroanisole has been used as a photoactive reagent to label a protein (bovine serum albumin), showing that under the irradiation conditions used, the label is incorporated into the polypeptide mainly through modification of epsilon-amino groups of the lysine residues.
描述了一种用于对具有生物学意义的化合物进行放射性标记的系统，这些化合物由于电子密度低，无法通过标准碘化技术进行标记。以对硝基苯甲醚为模型，我们通过用三氟乙酸铊处理制备了2-[¹²⁵I]碘-4-硝基苯甲醚，随后根据A. McKillop等人（《美国化学会志》93, 4841 - 4844 (1970)）的方法用碘离子取代铊。标记的碘硝基苯甲醚已被用作光活性试剂来标记一种蛋白质（牛血清白蛋白），表明在所使用的辐照条件下，该标记主要通过赖氨酸残基的ε-氨基的修饰而掺入到多肽中。
