Setchell K D, Lawson A M, McLaughlin L M, Patel S, Kirk D N, Axelson M
Biomed Mass Spectrom. 1983 Mar;10(3):227-35. doi: 10.1002/bms.1200100321.
Methods are described to extract and measure 2,3-bis(3-hydroxybenzyl)-gamma-butyrolactone (enterolactone) and 2,3-bis(3-hydroxybenzyl)butane-1,4-diol (enterodiol) from physiological fluids. They are based on the use of stable labelled analogues of each compound as internal standards with end point assay being by gas chromatography mass spectrometry. The methods were developed to quantify enterolactone and enterodiol in experiments designed to investigate the significance and source of these compounds in man and animals. Examples of studies on human ovarian blood and investigations of their excretion in urine following dietary manipulation are described.
本文描述了从生理体液中提取和测量2,3-双(3-羟基苄基)-γ-丁内酯（肠内酯）和2,3-双(3-羟基苄基)丁烷-1,4-二醇（肠二醇）的方法。这些方法基于使用每种化合物的稳定同位素标记类似物作为内标，终点测定采用气相色谱-质谱联用。这些方法是为了在旨在研究这些化合物在人和动物体内的意义及来源的实验中对肠内酯和肠二醇进行定量而开发的。文中描述了人体卵巢血液的研究实例以及饮食干预后尿液中这些物质排泄情况的调查。
