Westfall D P, Fedan J S, Colby J, Hogaboom G K, O'Donnell J P
Eur J Pharmacol. 1983 Mar 4;87(4):415-22. doi: 10.1016/0014-2999(83)90080-8.
In order to determine if ATP contributes as an excitatory transmitter in the guinea-pig bladder, experiments were conducted with ANAPP3, a photoaffinity analogue of ATP, which is an antagonist of adenine nucleotides in several other smooth muscles. With or without photoactivation with visible light, ANAPP3 antagonized contractile responses of in vitro strips of bladder to exogenous ATP. The antagonism was specific in that responses to acetylcholine and KCl were not affected by ANAPP3. Responses of strips of bladder to transmural electrical stimulation were not antagonized by ANAPP3 and were relatively insensitive to atropine. However, combined treatment with ANAPP3 and atropine produced a marked antagonism of the neurogenic response. In experiments with bladders obtained from animals pretreated with 6-hydroxydopamine, the ANAPP3-sensitive component of the neurogenic response was absent. These results suggest that acetylcholine, released from cholinergic nerves, and a purine, released from 6-hydroxy-dopamine-sensitive nerves, are both involved in motor transmission in this tissue.
为了确定三磷酸腺苷（ATP）是否作为豚鼠膀胱中的一种兴奋性递质发挥作用，研究人员使用了ANAPP3（一种ATP的光亲和类似物，它在其他几种平滑肌中是腺嘌呤核苷酸的拮抗剂）进行了实验。无论有无可见光光激活，ANAPP3均能拮抗膀胱体外条带对外源性ATP的收缩反应。这种拮抗作用具有特异性，因为对乙酰胆碱和氯化钾的反应不受ANAPP3影响。膀胱条带对跨壁电刺激的反应不受ANAPP3拮抗，且对阿托品相对不敏感。然而，ANAPP3与阿托品联合处理可显著拮抗神经源性反应。在用6-羟基多巴胺预处理的动物所获取的膀胱进行的实验中，神经源性反应中对ANAPP3敏感的成分缺失。这些结果表明，胆碱能神经释放的乙酰胆碱和6-羟基多巴胺敏感神经释放的一种嘌呤，均参与了该组织中的运动传递。
