斑点印迹法——一种用于杂交瘤培养物中抗体的新型筛选检测方法。
Dot-blotting--a novel screening assay for antibodies in hybridoma cultures.
作者信息
Sternberg J, Jeppesen P
出版信息
J Immunol Methods. 1983 Nov 11;64(1-2):39-43. doi: 10.1016/0022-1759(83)90382-4.
PMID:6644033
Abstract
A novel solid phase radioimmunoassay using nitrocellulose filters, is described. The method is of particular value in screening hybridoma cultures for monoclonal antibodies against chromosomal protein. Also it permits detection of antibodies directed against epitopes stable to SDS denaturation.
摘要
本文描述了一种使用硝酸纤维素滤膜的新型固相放射免疫测定法。该方法在筛选针对染色体蛋白的单克隆抗体的杂交瘤培养物中具有特殊价值。此外，它还能检测针对对SDS变性稳定的表位的抗体。
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