大鼠单克隆抗体。I. 从体外培养上清液中快速纯化。
Rat monoclonal antibodies. I. Rapid purification from in vitro culture supernatants.
作者信息
Bazin H, Xhurdebise L M, Burtonboy G, Lebacq A M, De Clercq L, Cormont F
出版信息
J Immunol Methods. 1984 Feb 10;66(2):261-9. doi: 10.1016/0022-1759(84)90337-5.
A technique for purifying rat monoclonal antibodies quickly and efficiently from in vitro culture supernatants is described. It is based on the fact that more than 95% of rat immunoglobulins carry kappa light chains. A mouse monoclonal antibody with suitable binding affinity for rat kappa light chains is immobilized on solid support and used to purify rat immunoglobulins. Milligrams of rat monoclonal antibodies may be rapidly concentrated from culture supernatants with high recovery. Rat monoclonal antibodies expressing lambda light chains (about 5% of the total) may be purified in a similar way with an appropriate anti-rat lambda chain monoclonal antibody.
本文描述了一种从体外培养上清液中快速高效纯化大鼠单克隆抗体的技术。该技术基于这样一个事实，即超过95%的大鼠免疫球蛋白携带κ轻链。将对大鼠κ轻链具有合适结合亲和力的小鼠单克隆抗体固定在固相支持物上，用于纯化大鼠免疫球蛋白。可以从培养上清液中快速浓缩出毫克级的大鼠单克隆抗体，回收率很高。表达λ轻链的大鼠单克隆抗体（约占总数的5%）可以用合适的抗大鼠λ链单克隆抗体以类似的方式进行纯化。
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