铜绿假单胞菌外毒素A：通过光亲和失活制备类毒素
Pseudomonas exotoxin A: toxoid preparation by photoaffinity inactivation.
作者信息
Marburg S, Tolman R L, Callahan L T
出版信息
Proc Natl Acad Sci U S A. 1983 May;80(10):2870-3. doi: 10.1073/pnas.80.10.2870.
Abstract
A method for toxoid preparation has been developed in which toxins expressing enzymatic activity can be detoxified by photoaffinity labeling techniques. In the case of Pseudomonas aeruginosa exotoxin A, the method relies on the affinity of azido-substituted analogs of the substrate (NAD) for the proenzyme form of the toxin. Photolysis of the putative toxin-analog complex results in irreversible inactivation of the toxin without loss of antigenic character. It is proposed that this occurs by nitrene insertion into a chemical bond on the toxin molecule. This affinity photoinactivation process should be applicable to other ADP-ribosylating toxins.
摘要
已开发出一种类毒素制备方法，其中可通过光亲和标记技术使具有酶活性的毒素解毒。对于铜绿假单胞菌外毒素A，该方法依赖于底物（NAD）的叠氮取代类似物对毒素酶原形式的亲和力。假定的毒素 - 类似物复合物的光解导致毒素不可逆失活，而不会丧失抗原特性。有人提出，这是通过氮烯插入毒素分子上的化学键来实现的。这种亲和光灭活过程应适用于其他ADP - 核糖基化毒素。
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本文引用的文献
1
PURINE NUCLEOSIDES. IX. THE SYNTHESIS OF 9-BETA-D-RIBOFURANOSYL URIC ACID AND OTHER RELATED 8-SUBSTITUTED PURINE RIBONUCLEOSIDES.嘌呤核苷。IX. 9-β-D-呋喃核糖基尿酸及其他相关8-取代嘌呤核糖核苷的合成。
J Am Chem Soc. 1965 Apr 20;87:1772-6. doi: 10.1021/ja01086a028.
2
Effect of formalin toxoiding on Pseudomonas aeruginosa toxin A: biological, chemical, and immunochemical studies.福尔马林类毒素处理对铜绿假单胞菌毒素A的影响：生物学、化学及免疫化学研究
Infect Immun. 1981 May;32(2):759-68. doi: 10.1128/iai.32.2.759-768.1981.
3
Expression of enzymic activity by exotoxin A from Pseudomonas aeruginosa.铜绿假单胞菌外毒素A的酶活性表达
Infect Immun. 1980 May;28(2):494-501. doi: 10.1128/iai.28.2.494-501.1980.
4
Photochemical labeling of yeast alcohol dehydrogenase with an azide analog of NAD+.用NAD⁺的叠氮类似物对酵母乙醇脱氢酶进行光化学标记。
Photochem Photobiol. 1973 Aug;18(2):135-8. doi: 10.1111/j.1751-1097.1973.tb06403.x.
5
Interactions of a photo-affinity ATP analog with cation-stimulated adenosine triphosphatases of human red cell membranes.一种光亲和性ATP类似物与人红细胞膜阳离子刺激的三磷酸腺苷酶的相互作用。
Proc Natl Acad Sci U S A. 1974 Sep;71(9):3367-71. doi: 10.1073/pnas.71.9.3367.
6
Use of microculture plates and the multiple automated sample harvester for in vitro microassay of bacterial toxins.使用微孔培养板和多重自动样品采集器进行细菌毒素的体外微量测定。
Appl Microbiol. 1974 Aug;28(2):326-7. doi: 10.1128/am.28.2.326-327.1974.
7
Photoaffinity labeling.
Methods Enzymol. 1977;46:69-114. doi: 10.1016/s0076-6879(77)46012-9.
8
Affinity labeling--an overview.亲和标记——综述
Methods Enzymol. 1977;46:3-14. doi: 10.1016/s0076-6879(77)46005-1.
9
Photoaffinity labeling of biological systems.生物系统的光亲和标记
Annu Rev Biochem. 1979;48:293-325. doi: 10.1146/annurev.bi.48.070179.001453.
10
NAD-dependent inhibition of protein synthesis by Pseudomonas aeruginosa toxin,铜绿假单胞菌毒素对蛋白质合成的NAD依赖性抑制作用
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2284-8. doi: 10.1073/pnas.72.6.2284.
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