Marburg S, Tolman R L, Callahan L T
Proc Natl Acad Sci U S A. 1983 May;80(10):2870-3. doi: 10.1073/pnas.80.10.2870.
A method for toxoid preparation has been developed in which toxins expressing enzymatic activity can be detoxified by photoaffinity labeling techniques. In the case of Pseudomonas aeruginosa exotoxin A, the method relies on the affinity of azido-substituted analogs of the substrate (NAD) for the proenzyme form of the toxin. Photolysis of the putative toxin-analog complex results in irreversible inactivation of the toxin without loss of antigenic character. It is proposed that this occurs by nitrene insertion into a chemical bond on the toxin molecule. This affinity photoinactivation process should be applicable to other ADP-ribosylating toxins.
已开发出一种类毒素制备方法，其中可通过光亲和标记技术使具有酶活性的毒素解毒。对于铜绿假单胞菌外毒素A，该方法依赖于底物（NAD）的叠氮取代类似物对毒素酶原形式的亲和力。假定的毒素 - 类似物复合物的光解导致毒素不可逆失活，而不会丧失抗原特性。有人提出，这是通过氮烯插入毒素分子上的化学键来实现的。这种亲和光灭活过程应适用于其他ADP - 核糖基化毒素。
